不同含水率对谷子籽粒压缩力学性质与摩擦特性的影响

为了探明不同含水率谷子籽粒的物理机械性质,减少谷子籽粒在播种、碾米加工及储运等过程中受到压缩载荷及摩擦而产生的机械损伤,该文针对不同含水率的谷子籽粒进行压缩力学性质与摩擦特性试验。研究了谷子籽粒的挤压破碎过程,获得不同含水率谷子籽粒的力-位移（变形）曲线,破坏力、变形量及破坏能。随着含水率升高,破坏力减小,变形量和破坏能呈现先降低后升高的变化规律。同时采用赫兹接触理论,得到谷子籽粒单向表观弹性模量和许用挤压应力,结果表明二者都随含水率升高线性降低。分别测定了谷子籽粒与钢板和铝板间的滑动摩擦系数,随含水率升高,谷子与该2种材料的摩擦系数均增大,且与铝板的摩擦系数要高于钢板。根据试验结果,分别拟合得到了压缩和摩擦力学性能指标与谷子含水率的关系方程,为谷子播种、仓储、加工等装备设计及参数优化提供了基础依据。     

Development of Core Subset of Finger Millet Germplasm Using Geographical Origin and Data on 14 Quantitative Traits

Finger millet [Eleusine coracana (L.) Gaertn.] is an important cereal food crop in Africa and South Asia. It is a hardy crop that can be grown in very diverse environments from almost at sea level to about 2400 m.a.s.l. Finger millet has an excellent food value as its seeds contain protein ranging from 7 to 14% and are particularly rich in methionine amino acid, iron, and calcium. Despite all these merits, this crop has been neglected from the main stream of crop improvement research. One of the means to boost its production and productivity is to enhance utilization of finger millet germplasm to breed superior varieties. Keeping this objective in view, a core subset of finger millet germplasm (622 accessions) based on origin and data on 14 quantitative traits was developed from the entire global collection of 5940 accessions held in the genebank at ICRISAT, Patancheru, India. The comparison of means, variances, frequency distribution, Shannon-Weaver diversity index (H′) and phenotypic correlations indicated that the core subset represents the entire collection. These tests indicated that sampling was optimal and the diversity has been captured very well in the core subset. The correlation analysis indicated that panicle exsertion and longest finger length could be given lower priority in the future germplasm evaluation work of finger millet.     

水分胁迫下糜子籽粒淀粉合成关键酶及与灌浆特性的关联

以‘榆糜2号’为材料,采用盆栽试验,以正常供水为对照,研究拔节期中度和重度水分胁迫、抽穗期中度和重度水分胁迫对糜子籽粒淀粉合成关键酶活性变化和籽粒灌浆特性。结果表明:水分胁迫抑制糜子籽粒灌浆过程中的淀粉分支酶(Q酶)、蔗糖合成酶(SS)、ADP-葡萄糖焦磷酸化酶(AGPP)3种酶活性,不同胁迫程度相比,重度水分胁迫对淀粉合成关键酶活性影响较大,中度水分胁迫次之;不同胁迫时期相比,拔节期水分胁迫对淀粉合成关键酶活性影响较大,抽穗期水分胁迫次之。3个酶的变化与籽粒灌浆动态相关联,Q酶和蔗糖合成酶活性峰值出现的时间在糜子籽粒最大灌浆速率出现的时间之后,AGPP最高活性出现的时间提前或同步于最大灌浆速率的时间。相关分析表明,Q酶、SS和AGPP活性与平均灌浆速率和最大灌浆速率均呈显著或极显著正相关。可见,水分胁迫使糜子籽粒淀粉合成关键酶活性受到抑制,从而导致籽粒灌浆速率和产量降低。     

谷子显性恢复基因的AFLP分析

赤峰显性核不育谷子是在谷子中首次发现的核不育材料,该材料的育性受2对核显性基因互作控制,一对是显性核不育基因Msch,另一对是显性上位育性恢复基因Rf。两者共同存在时显性上位育性恢复基因Rf能抑制显性核不育基因Msch的表达,从而表现可育。利用已构建的不育基因Msch的上位育性恢复基因Rf的近等基因系(NILs)为材料,通过对300对AFLP引物组合进行筛选,找到了与显性上位育性恢复基因Rf紧密连锁的2个AFLP标记(E15/M52和E20/M41),与不育基因的遗传距离分别是7.0c M和12.7 c M,而且位于不育基因的同一侧,标记间相距5.7 c M。     

Enzymes of Ammonia Assimilation and Transamination in Relation to Hybrid Vigour in Pearl Millet (Pennisetum americanum L. Leeke)

Changes in the activities of enzymes of ammonia assimilation viz., glutamate dehydrogenase (GDH), glutamine synthetase (GS) and glutamate synthase (GOGAT), together with the activities of glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) were analysed in the topmost fully expanded leaf of pearl millet (Pennisetum americanum L. Leeke) hybrid BJ-104 , and its parents, J-104 and 5141-A , during the entire period of growth in field. Ammonia assimilation enzymes showed strong ontogenic trends and the hybrid attained activities either equal to the better (male) parent or showed better parental heterosis. In contrast to this both GOT and GPT activities were slightly higher than the poor (female) parent. The role of these enzymes in relation to chlorophyll synthesis and transport of metabolites from cellular compartments of C₄ plants, are discussed. Surveying the literature on attempts to propose biochemical criteria for hybrid vigour, it is proposed that there cannot be a universal criterion, rather rate limiting steps in different cultivars should be searched.     

Unsaturated fatty acids from zoospores of <Emphasis Type="Italic">Sclerospora graminicola</Emphasis> induce resistance in pearl millet

Downy mildew of pearl millet, caused by Sclerospora graminicola, is a devastating disease, resulting in high economic losses in the semi-arid regions of the world. Recently, induction of host plant resistance using biotic and abiotic inducers are included among disease management practices as an eco-friendly approach. Unsaturated fatty acids are considered as a new generation of plant disease resistance inducers. In the present study, six unsaturated fatty acids, viz. docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), arachidonic acid (AA), linolenic acid, linoleic acid and oleic acid, all originally detected in the zoospores of S. graminicola,were applied to seeds of susceptible cultivars of pearl millet to examine their ability to protect against downy mildew under greenhouse and field conditions. In greenhouse experiments, EPA and AA induced a maximum of 78.6% and 76.5% protection, whereas linoleic acid, DHA and linolenic acid provided up to 66.3%, 61.2% and 24.5% protection, respectively. Oleic acid was not effective in protecting pearl millet (only 5.1% protection). A time interval of four days between treatment of seeds and challenge inoculation was required to obtain optimum protection. Plants raised from treated seeds and challenge inoculated at the tillering and inflorescence stages showed enhanced resistance, resulting in higher grain yield compared to untreated plants of the same cultivar. Chitinase activity was found to be higher in susceptible seedlings of pearl millet after treatment with the fatty acids and pathogen inoculation than in seedlings only inoculated with the pathogen. This indicates that host defence responses are activated and thus that induced resistance is involved in the protection observed. The role of unsaturated fatty acids as activators of resistance against downy mildew in pearl millet is discussed.     

Note: Proline — An inducer of resistance against pearl millet downy mildew disease caused by<Emphasis Type="Italic">Sclerospora graminicola</Emphasis>

In an attempt to find a suitable alternative to the otherwise perilous chemical control strategy of disease management, the amino acid proline was evaluated for its efficiency to elicit resistance in pearl millet (Pennisetum glaucum (L.) R. Br.) against downy mildew disease caused bySclerospora graminicola (Sacc.) Schroet both under greenhouse and field conditions. Proline treatment to seeds at 50 mM concentration for 3 h, significantly enhanced the seed germination and seedling vigor of pearl millet in comparison with the control. The same concentration and duration of seed treatment protected the pearl millet plants from downy mildew by offering 58% protection under greenhouse and 67% protection under field conditions. Studies revealed that 3 days were required for proline-treated plants to develop resistance, which was systemic and was sustained throughout the life of the plants. Apart from disease protection, proline was also found effective in enhancing vegetative and reproductive growth of the plants, as evidenced by the increase in height, fresh weight, leaf area, tillering capacity, 1000-seed weight and grain yield in comparison with the control plants. http://www.phytoparasitica.org posting Oct. 3, 2004.     

基施尿素对片麻岩新成土旱地谷子生长及土壤硝态氮时空分布的影响

本试验在片麻岩新成山地土壤上,设置0(CK)、N 120kg/hm2(N120)、N 225 kg/hm2(N225)、N 300kg/hm2(N300)4个施氮水平,布置田间微区试验,小区面积为10m2,研究了尿素不同施用量对旱地谷子生长及土壤硝态氮时空分布的影响。定位试验第二年的结果表明:在该试验条件下,N120处理谷子产量达到最高为4.76 kg/10m2,之后提高施肥量N225和N300处理的谷子产量并没有显著增加;从0~60 cm土壤剖面中硝态氮时空分布的差异可以看出:在施肥20 d后,N120、N225和N300处理NO3--N含量在0~40 cm土层显著增加,其中N225和N300处理NO3--N已经下移到40~60 cm土层。施肥80 d后,各施肥处理的硝态氮有一部分已经移出60 cm土层。到施肥96 d(谷子收获),N120、N225和N300处理比CK处理土壤剖面中NO3--N含量显著增加,且氮肥用量越高,土壤中NO3--N含量越高。     

覆膜方式对旱地冬小麦产量和水分利用率的影响

研究了不同覆膜方式对旱作区冬小麦产量和水分利用率的影响。结果表明,在生育期降水量200.0mm下,全膜小垄沟穴播处理冬小麦平均折合产量4 615.4 kg/hm2,较露地条播增产42.9%,总产值、纯收入较露地条播分别增加3 599.9、1 998.1元/hm2;降水利用率64.5%,较露地条播降低0.9百分点;土壤水分利用率15.0 kg(/mm.hm)2,较露地条播增加5.0 kg(/mm.hm)2。     

抗除草剂谷子杂交种长杂2号高产高效栽培技术研究

采用正交设计,对长杂2号的播期、施肥、留苗密度、种植方式4个因素的栽培技术进行了试验。结果表明,长杂2号在山西省中晚熟区取得高产的栽培方案是:最适播期5月20—25日,公顷留苗30万株左右,采用宽窄行种植方式。